I have made a persistent live stick with Ubuntustudio 20.04 from ubuntustudio-20.04.2.0-dvd-amd64.iso.
I applied some changes to it (e.g. install Chromium and uninstall Firefox, because it caused memory freeze on a Lenovo T430 laptop in Jitsi meetings with camera enabled). Yeah: it took an awful lot of time installing and uninstalling software (I had to let it run over night) on a thumbdrive with persistence, although I used an USB3-device.
Now I have almost reached my target. But I noticed a message booting from that pendrive (it was not there in the precedent bootings from it)
./boot/grub/x86_64-efi/div.mod: OK Checking ./boot/grub/loopback.cfg ./boot/grub/loopback.cfg: OK
Check finished: errors found in 1 files! You might encounter errors.
The system booted despite that message. Unfortunately the time consumption for booting has grown to almost 4 minutes. Initially it was less than 2 minutes!
There has always been a file md5sums.txt at the root of my thumbdrive. From another system, I went to the directory at the root of the thumbdrive and then applied md5sum -c md5sums.txt. According to the output of that, they are all ok.
How can I find out for which file the complaint at boot time was?
BTW: When shutting down the computer booted from the persistent live drive, lots of file IO happens in the partition casper-rw. Unfortunately after clicking on the button to really shut down the computer, the screen immediately blanks and only a tiny cursor is in the left upper corner of the screen which might easily get overlooked. It looks like this is when casper-rw is updated. Depending on what one has done before, this "dark phase" before the Ubuntustudio logo with that rotating indicator can take up to one minute! It would be nice to display a warning against pulling out the thumbdrive all the time, when casper-rw is updated! Otherwise the persistent stick is easily ruined.