I guess the answer is no.
Take an observer as a geodesic, seems its nature to choose his space as the space "spanned" by the basis vectors which are orthogonal to the time basis (the tangent vector of the geodesic).
However, for curved manifold, the previous chosen basis vector can only span a subspace of tangent space, but not a region on the manifold.
If we consider a region which is almost Minkowskian only, we can define the concept of space there, but what if we exclude it?
So whether there exists some nature definition of space in GR?
If the answer it no, how we build up our reference frame physically in GR? Or even the definition of reference frame just works in the Minkowskian-like region?