The potential difference
Finding the potential difference is a bit more complicated than you suggested, if I interpreted the meaning of $k$, $q$ and $r$ in your formula correctly.
Before connecting the spheres, they effectively form a capacitor with some capacitance $C$. If $r_1 = r_2$, you can use a formula from wikipedia to calculate $C$, which then gives you the voltage (potential difference) as
$$
V = \frac 1C \frac{q_1+q_2}{2}~.
$$
If $r_1 \neq r_2$, you will have to solve the Poisson equation,
$$
\Delta \phi(\vec r) = - \frac{\rho(\vec r)}{\varepsilon_0}~,
$$
where $\phi$ is the electrical potential, $\rho$ is the charge density, $\vec r$ is the position, $\Delta$ is the Laplacian and $\varepsilon_0$ is the dielectric constant of the vacuum. The total charge on each of the spheres serves as boundary condition for the solution. As soon as a solution $\phi(\vec r)$ is found, the potential difference can directly be calculated.
The electric current
Once the voltage is known, the current is, by Ohm's law, simply
$$
I = \frac VR~,
$$
just as you stated. However this only holds immediately upon connection of the spheres. Afterwards, it makes little sense to assume the charges remain constant, because electric current is just a flow of charge which will eventually cause equal charge density on both spheres and the wire. This is nothing else than the discharge process of a capacitor, which can be modeled quite easily:
- Let $t$ be the time and $t = 0$ the point at which the spheres are connected. Now the current $I(t)$, the charge $Q(t)$ and the voltage $V(t)$ vary over time.
- Using Ohm's law, we have $V(t) = I(t) R$ and per definition of the capacitance it holds $V(t) = Q(t) / C$. This means
$$
\frac{Q(t)}{C} = I(t) R \qquad \Leftrightarrow \qquad \frac{Q(t)}{C} - I(t) R = 0~.
$$
- The current $I(t)$ is a change (the derivative) of charge, so $I(t) = \partial_t Q(t)$ and we have
$$
\frac{Q(t)}{C} - R \partial_t Q(t) = 0 \qquad \Leftrightarrow \qquad Q(t) - RC \partial_t Q(t) = 0~.
$$
- The solution of this first-order linear ordinary differential equation is
$$
Q(t) = Q(0) e^{-RCt}~,
$$
which is a parametrization of exponential decay.