Yayoi is a pre-historical era in Japan.
Yayoi can also refer to:
- Yayoi (given name), a Japanese female given name
 - Yayoi Kusama, a Japanese artist and writer
 - Yayoi people, an ancient ethnic group
 - Yayoi, Ōita, a town in Japan
 - Yayoi, Tokyo, an area of Tokyo
 - Japanese destroyer Yayoi, two destroyers
 - Yayoi Sho, a Japanese horse race
 
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