Virgo may refer to:
- Virgo (astrology), the sixth astrological sign of the zodiac
 - Virgo (constellation), a constellation
 - Virgo Cluster, a cluster of galaxies in the constellation Virgo
 - Virgo Stellar Stream, remains of a dwarf galaxy
 - Virgo Supercluster, a galactic supercluster
 - Virgo interferometer, a European gravitational-wave telescope
 
Arts
- Virgo (film), a 1970 Egyptian film
 - Virgo (comics), a Marvel Comics character
 - Virgos Merlot, or Virgos, an American rock band
 - Virgo (album), by Virgo Four, 1989
 - "Virgo", a song by Atmosphere from Mi Vida Local, 2018
 
Other uses
- Virgo (moth), a genus of moth
 - Virgo (software), an open source Java application server
 - Virgo (surname)
 - Aqua Virgo, one of the 11 aqueducts supplied the city of ancient Rome
 - SuperStar Virgo, a mega cruise ship operated and owned by Star Cruises
 - USS Virgo (AKA-20), a United States Navy attack cargo ship
 - Virgin Mary (Latin: Maria Virgo)
 
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