The following highways are numbered 53:

Cambodia

• National Road 53 (Cambodia)

Canada

• Alberta Highway 53

India

• National Highway 53 (India)

Ireland

• N53 road (Ireland)

Italy

• Autostrada A53

Japan

• Japan National Route 53

Korea, South

• National Route 53 (South Korea)

Mexico

• Mexican Federal Highway 53

New Zealand

• New Zealand State Highway 53

Norway

• Norwegian County Road 53 (Bokmål: Fylkesvei 53, Nynorsk: Fylkesveg 53)

Philippines

• N53 highway (Philippines)

Turkey

• O-53, a motorway in Turkey connecting O-52 with İskenderun, Hatay Province.

United Kingdom

• British A53 (Shrewsbury-Buxton)
• British M53 (Wallasey-Chester)

United States

• Interstate 53 (former proposal)
• U.S. Route 53
• Alabama State Route 53
• Arkansas Highway 53
• California State Route 53
• Colorado State Highway 53
• Connecticut Route 53
• Florida State Road 53
  • County Road 53 (Lafayette County, Florida)
  • County Road 53 (Madison County, Florida)
• Georgia State Route 53
• Idaho State Highway 53
• Illinois Route 53
• Indiana State Road 53
• Iowa Highway 53 (1926-1949) (former)
• K-53 (Kansas highway)
• Kentucky Route 53
• Louisiana Highway 53
  • Louisiana State Route 53 (former)
• Maryland Route 53
• Massachusetts Route 53
• M-53 (Michigan highway)
• County Road 53 (Anoka County, Minnesota)
  • County Road 53 (Hennepin County, Minnesota)
  • County Road 53 (Ramsey County, Minnesota)
• Mississippi Highway 53
• Missouri Route 53
• Nebraska Highway 53
  • Nebraska Link 53A
  • Nebraska Link 53B
  • Nebraska Link 53C
  • Nebraska Link 53E
• Nevada State Route 53 (former)
• New Jersey Route 53
  • County Route 53 (Bergen County, New Jersey)
  • County Route 53 (Monmouth County, New Jersey)
  • County Route 53 (Ocean County, New Jersey)
• New Mexico State Road 53
• New York State Route 53
  • County Route 53 (Cattaraugus County, New York)
  • County Route 53 (Chautauqua County, New York)
  • County Route 53 (Delaware County, New York)
  • County Route 53 (Dutchess County, New York)
  • County Route 53 (Franklin County, New York)
  • County Route 53 (Greene County, New York)
  • County Route 53 (Jefferson County, New York)
  • County Route 53 (Madison County, New York)
  • County Route 53 (Monroe County, New York)
  • County Route 53 (Montgomery County, New York)
  • County Route 53 (Oneida County, New York)
  • County Route 53 (Onondaga County, New York)
    • County Route 53A (Onondaga County, New York)
  • County Route 53 (Orange County, New York)
  • County Route 53 (Orleans County, New York)
  • County Route 53 (Otsego County, New York)
  • County Route 53 (Putnam County, New York)
  • County Route 53 (Rensselaer County, New York)
  • County Route 53 (Rockland County, New York)
  • County Route 53 (Saratoga County, New York)
  • County Route 53 (Suffolk County, New York)
  • County Route 53 (Sullivan County, New York)
  • County Route 53 (Ulster County, New York)
    • County Route 53A (Ulster County, New York)
  • County Route 53 (Warren County, New York)
  • County Route 53 (Westchester County, New York)
  • County Route 53 (Wyoming County, New York)
• North Carolina Highway 53
• North Dakota Highway 53
• Ohio State Route 53
• Oklahoma State Highway 53
  • Oklahoma State Highway 53A
• Oregon Route 53
• Pennsylvania Route 53
• South Carolina Highway 53
• South Dakota Highway 53
• Tennessee State Route 53
• Texas State Highway 53
  • Texas State Highway Spur 53
  • Farm to Market Road 53 (former)
  • Texas Park Road 53 (former)
• Utah State Route 53
• Vermont Route 53
• Virginia State Route 53
• West Virginia Route 53
  • West Virginia Route 53 (1920s) (former)
• Wisconsin Highway 53 (former)

Territories:

• Puerto Rico Highway 53