Jot or JOT may refer to:
Persons with the name
- Jot Agyeman (born 1967), Ghanaian actor
 - Jot D. Carpenter (1938–2000), American landscape architect
 - Jot Goar (1870–1947), American baseball player
 
Computing
- Jot (programming language)
 - The Journal of Object Technology
 - The Joy of Tech, a webcomic
 - Jot, a handwriting recognition system which became the basis for Palm OS' Graffiti 2
 
Other uses
- Jot, or Jat, an umbrella term for the peripatetic groups of Afghanistan
 - Jot (interval), a musical pitch interval
 - Jot (letter), or yot, a name for the letter J
 - JOT (TV series), a syndicated, animated television series
 - Jot, the Greek letter iota, in the stock phrase "not one jot or tittle"
 - Joliet Regional Airport (IATA: JOT)
 
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