Holston may refer to:
- Holston, Virginia
- Holston River
- Holston Formation
- Treaty of Holston
- Holston Conference, an annual conference within Methodism
- Holston Army Ammunition Plant in Kingsport, Tennessee
- The Holston, an NRHP-listed high-rise in Knoxville, Tennessee
People with the surname
- David Holston (born 1986), American basketball player
- Mike Holston (born 1958), American football player
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