Bernheim is a surname. Notable people with the surname include:
- Alain Bernheim (born 1931), French Masonic author
 - Alain Bernheim (producer) (1922–2009), French-born American film producer and literary agent
 - Emile Bernheim (1886–1985), Belgian industrialist
 - Emmanuèle Bernheim (1955–2017), French writer
 - Ernst Bernheim (1850–1942), German-Jewish historian
 - Erwin Bernheim (1925–2007), Swiss founder of Mondaine Watch Ltd.
 - Hippolyte Bernheim (1837–1919), French Jewish physician and neurologist
 - Gilles Bernheim (born 1952), chief rabbi of France 2009–2013
 - Isaac Wolfe Bernheim (1848–1945), Jewish distiller and philanthropist, founder of the I. W. Harper bourbon brand and the Bernheim Arboretum and Research Forest
 - Louis Bernheim (1861–1931), Belgian general
 - Mary Bernheim (Hare) (1902–1997), British-American biochemist
 
See also
- Bernheim petition, 1933 petition leading to the vacation of Nazi anti-Jewish legislation in German Upper Silesia until 1937.
 - 3467 Bernheim, an asteroid
 - Bernheim Arboretum and Research Forest, a park located twenty miles south of Louisville, Kentucky
 - Bernheim-Jeune, Art gallery and publisher in Paris
 
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