Ambrosio may refer to:
People
- Alessandra Ambrosio (born 1981), a Brazilian model
 - Arturo Ambrosio (1870–1960), an Italian film producer
 - Fabrisia Ambrosio, Brazilian-born physical therapist and academic
 - Franco Ambrosio (1932–2009), an Italian businessman
 - Frank Ambrosio (born 1949), American philosopher
 - Gabriele Ambrosio (1844–1918), Italian sculptor
 - Giovanni Ambrosio (1420–1484), Italian dancer and writer on dance (Guglielmo Ebreo da Pesaro)
 - Luigi Ambrosio (born 1963), an Italian mathematician
 - Marco Ambrosio (born 1973), Italian former footballer
 - Thomas Ambrosio (born 1971), American political scientist
 - Vittorio Ambrosio (1879–1958), an Italian general from World War I and World War II
 
Other
- Ambrosio (horse), a racehorse
 - Ambrosio Film, an Italian film production company that existed from 1906 to 1924
 
See also
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